Posts Tagged ‘Tumor Cells’

WEST LAFAYETTE, Ind. — The pawpaw (Graviola) tree, which bears the largest fruit native to North America, may bear new fruit forscientists seeking ways to fight cancer. Purdue University researcher Jerry McLaughlin, working with doctoral student Nicholas Oberlies, has found compounds in the bark of the tree that have shown preliminary success in fighting some drug-resistant cancers.The studies, published in two separate journal articles this summer, show that the pawpaw compounds not only are effective in killing tumors that have proven resistant to anti-cancer agents, but also seem to have a special affinity for such resistant cells.

The findings were detailed in the journal Cancer Letters and the Journal of Medicinal Chemistry. Though further studies are needed to pinpoint exactly how the pawpaw compounds work within the cancer cell, McLaughlin says their effect is to pull the plug on the energy-producing mechanisms in the cell. McLaughlin notes, however, that the effect on drug-resistant cells has been studied only in laboratory cultures and will require additional study in animals before it can be tested in humans.”Multidrug-resistant cancer is hard to treat because the cancer cell has developed a mechanism to get around the anti-cancer agent,” says McLaughlin, professor of pharmacognosy in Purdue’s School of Pharmacy and Pharmacal Science. “Tumor cells that survive chemotherapy treatments often recover with increased resistance to the agent used in the original treatment program as well as to other related drugs.”

Such resistance can develop when surviving cancer cells develop one or more mechanisms to accelerate the removal of noxious substances, including anti-cancer drugs. One of the most common mechanisms used to circumvent the anti-cancer agents is to develop a “pump” that is capable of pushing anti-cancer agents out of the cell before they can kill it. These pumps are called P-glycoprotein mediated pumps and are named for the type of protein used to construct and operate them. Though all cells have the ability to develop such a pump, normal cells seldom do. Even in cancer cells, which do not respond normally to the body’s control mechanisms, only a small percentage of cells develop this pumping mechanism. “If having this pump was such a good deal, all cells would have it. But all cells don’t,” McLaughlin says. “In a given population of cancer cells in a person, maybe only 2 percent of the cancer cells possess this pump. But it’s those 2 percent of cancer cells that eventually grow and expand to create drug-resistant tumors.”

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One of the tricks currently attempted in treating cancer patients is to flood the body with other compounds to keep the pump busy, and then administer high doses of an anti-cancer agent in hopes that some of it will be able to stay in long enough to kill the cancer cell. “But the high doses of the drugs required for this treatment often produce side effects, such as loss of blood pressure, so the patient often succumbs to the side effects of the treatment,” McLaughlin says.Though this pump mechanism is efficient at eliminating most anti-cancer agents, McLaughlin, whose research group has identified more than 40 pawpaw compounds with anti-cancer properties, discovered a series of the compounds, called Annonaceous acetogenins, that were capable of killing cancer cells that employed this mechanism. He then designed a laboratory study to analyze the cytotoxic or cell-killing effects of one of the compounds, called bullatacin, on human mammary cancer cells. The study compared bullatacin’s effects on standard, nonresistant cancer cells and on multidrug-resistant cells.In the June issue of Cancer Letters, the research team reported that bullatacin prefere intially killed the multidrug-resistant cells by inhibiting the production of adenosine triphosphate, or ATP. ATP is a compound that works to release energy in acell and is essential to all cell processes. “A multidrug-resistant cell requires a tremendous amount of energy to run the pump and extrude things out of the cell,” McLaughlin says. “By inhibiting ATP production, we’re essentially pulling the plug on its energy source.”Though the pawpaw compounds also inhibited ATP production in noncancerous cells and nonresistant cancer cells, those cells were not affected as dramatically, McLaughlin says. Pawpaw shows promise in fighting drug-resistant tumors file: Purdue University News

“Normal cells and standard cancer cells may be able to minimize the effects of this compound because they don’t require the vast amounts of energy needed by the pump-running cells,” McLaughlin says. “The resistant cell is using its extra energy for this pump as well as to grow, so it is really taxed for energy. When we mess with the energy supply, it kills the cell.” McLaughlin and his group then did a follow-up study to test a series of 14 structurally similar pawpaw compounds to determine the structural features that maximize this biological activity in multidrug-resistant cancer cells. The results were published in the June issue of the Journal of Medicinal Chemistry. “This study tells us how to maximize this activity, so we have a pretty good idea what compounds we’d like to try in animals with multidrug-resistant tumors,” McLaughlin says. If proven effective in animals and humans, McLaughlin says, the compounds may be used to treat multidrug resistance in a variety of cancers, because many types of cancer cells develop resistance by employing a pump.The studies were funded by National Institutes of Health/National Cancer Institute, the Indiana Elks Cancer Research Fundantion, Purdue Research Foundation. Purdue has filed a patent on the use of the pawpaw compounds.

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Free Tecnical Report of Graviola Source: Jerry McLaughlin, (765) 494-1455; e-mail, jac@pharmacy.purdue.edu Writer: Susan Gaidos, (765) 494-2081; e-mail, susan_gaidos@uns.purdue.edu Purdue News Service: (765) 494-2096; e-mail, purduenews@uns.purdue.edu ABSTRACT: Cancer Letters 115 (1997) 73-79 The Annonaceous acetogenin bullatacin is cytotoxic against multidrug-resistant human mammary adenocarcinoma cells Nicholas H. Oberlies, Vicki L. Croy, Marietta L. Harrison, Jerry L. McLaughlinDepartment of Medicinal Chemistry and Molecular Pharmacology, School of Pharmacy and Pharmacal Sciences, Purdue University.Cytotoxic effects of the Annonaceous acetogenin, bullatacin, were studied in multidrug-resistant (MDR) human mammary adenocarcinoma (MCF-7/Adr) cells vs. the parental non-resistant wild type (MCF-7/wt) cells. Bullatacin was effectively cytotoxic to the MCF-7/Adr cells while it was more cytostatic to the MCF-7/wt cells. ATP depletion is the mode of action of the Annonaceous acetogenins, and these agents offer a special advantage in the chemotherapeutic treatment of MDR tumors that have ATP-dependent mechanisms. ABSTRACT: J. Med. Chem. 1997, 40, 2102-2106 Structure-activity relationships of diverse Annonaceous acetogenins against multidrug-resistant human mammary adenocarcinoma (MCF-7/Adr) cells Nicholas H. Oberlies, Ching-jer Chang, Jerry L. McLaughlin Department of Medicinal Chemistry and Molecular Pharmacology, School of Pharmacy and Pharmacal Sciences, Purdue University. Fourteen structurally diverse Annonaceous acetogenins, representing the three main classes of bis-adjacent, bis-nonadjacent, and single-THF ring(s), were tested for their ability to inhibit the growth of adriamycin-resistant human mammary adenocarcinoma (MCF-7/Adr) cells. This cell line is resistant to treatment with adriamycin, vincristine, and vinblastine and is, thus, multidrug-resistant (MDR). Among a series of bis-adjacent THF ring acetogenins, those with the stereochemistry of threo-trans-threo-trans-erythro (from C-15 to C-24) were the most potent with as much as 250 times the potency of adriamycin. A spacing of 13 carbons between the flanking hydroxyl of the THF ring system and the gamma-unsaturated lactone seems to be optimum with a spacing of 11 and 9 carbons being significantly less active. Several single-THF ring compounds were also quite potent, with gigantetrocin A (11) being the most potent compound tested. The acetogenins may, thus, have chemotherapeutic potential, especially with regard to MDR tumors.

“10000 times stronger killer of Cancer than Chemo”.. do share it.. can save many lives, fill up hopes and build confidence in the patients..

The SourSop or the fruit from the graviola tree is a miraculous natural cancer cell killer 10,000 times stronger than Chemo.

Why are we not aware of this? It’s because some big corporation want to make back their money spent on years of research by trying to make a synthetic version of it for sale.

So, since you know it now you can help a friend in need by letting him know or just drink some soursop juice yourself as prevention from time to time. The taste is not bad after all. It’s completely natural and definitely has no side effects. If you have the space, plant one in your garden. The other parts of the tree are also useful. The next time you have a fruit juice, ask for a sour sop.

How many people died in vain while this billion-dollar drug maker concealed the secret of the miraculous Graviola tree? This tree is low and is called graviola in Brazil, guanabana in Spanish and has the uninspiring name “soursop” in English. The fruit is very large and the subacid sweet white pulp is eaten out of hand or, more commonly, used to make fruit drinks, sherbets and such.

The principal interest in this plant is because of its strong anti-cancer effects. Although it is effective for a number of medical conditions, it is its anti tumor effect that is of most interest. This plant is a proven cancer remedy for cancers of all types.

Besides being a cancer remedy, graviola is a broad spectrum antimicrobial agent for both bacterial and fungal infections, is effective against internal parasites and worms, lowers high blood pressure and is used for depression, stress and nervous disorders.

If there ever was a single example that makes it dramatically clear why the existence of Health Sciences Institute is so vital to Americans like you, it’s the incredible story behind the Graviola tree.

The truth is stunningly simple: Deep within the Amazon Rainforest grows a tree that could literally revolutionize what you, your doctor, and the rest of the world thinks about cancer treatment and chances of survival. The future has never looked more promising.

Research shows that with extracts from this miraculous tree it now may be possible to: * Attack cancer safely and effectively with an all-natural therapy that does not cause extreme nausea, weight loss and hair loss * Protect your immune system and avoid deadly infections * Feel stronger and healthier throughout the course of the treatment * Boost your energy and improve your outlook on life

The source of this information is just as stunning: It comes from one of America ‘s largest drug manufacturers, the fruit of over 20 laboratory tests conducted since the 1970′s! What those tests revealed was nothing short of mind numbing… Extracts from the tree were shown to:

* Effectively target and kill malignant cells in 12 types of cancer, including colon, breast, prostate, lung and pancreatic cancer. * The tree compounds proved to be up to 10,000 times stronger in slowing the growth of cancer cells than Adriamycin, a commonly used chemotherapeutic drug! * What’s more, unlike chemotherapy, the compound extracted from the Graviola tree selectively hunts down and kills only cancer cells. It does not harm healthy cells!

The amazing anti-cancer properties of the Graviola tree have been extensively researched–so why haven’t you heard anything about it? If Graviola extract is as half as promising as it appears to be–why doesn’t every single oncologist at every major hospital insist on using it on all his or her patients?

The spine-chilling answer illustrates just how easily our health–and for many, our very lives(!)–are controlled by money and power.

Graviola–the plant that worked too well

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One of America’s biggest billion-dollar drug makers began a search for a cancer cure and their research centered on Graviola, a legendary healing tree from the Amazon Rainforest.

Various parts of the Graviola tree–including the bark, leaves, roots, fruit and fruit-seeds–have been used for centuries by medicine men and native Indians in South America to treat heart disease, asthma, liver problems and arthritis. Going on very little documented scientific evidence, the company poured money and resources into testing the tree’s anti-cancerous properties–and were shocked by the results. Graviola proved itself to be a cancer-killing dynamo.

But that’s where the Graviola story nearly ended.

The company had one huge problem with the Graviola tree–it’s completely natural, and so, under federal law, not patentable. There’s no way to make serious profits from it.

It turns out the drug company invested nearly seven years trying to synthesize two of the Graviola tree’s most powerful anti-cancer ingredients. If they could isolate and produce man-made clones of what makes the Graviola so potent, they’d be able to patent it and make their money back. Alas, they hit a brick wall. The original simply could not be replicated. There was no way the company could protect its profits–or even make back the millions it poured into research.

As the dream of huge profits evaporated, their testing on Graviola came to a screeching halt. Even worse, the company shelved the entire project and chose not to publish the findings of its research!

Luckily, however, there was one scientist from the Graviola research team whose conscience wouldn’t let him see such atrocity committed. Risking his career, he contacted a company that’s dedicated to harvesting medical plants from the Amazon Rainforest and blew the whistle.

Miracle unleashed

When researchers at the Health Sciences Institute were alerted to the news of Graviola, they began tracking the research done on the cancer-killing tree. Evidence of the astounding effectiveness of Graviola–and its shocking cover-up–came in fast and furious….

….The National Cancer Institute performed the first scientific research in 1976. The results showed that Graviola’s “leaves and stems were found effective in attacking and destroying malignant cells.” Inexplicably, the results were published in an internal report and never released to the public…

…Since 1976, Graviola has proven to be an immensely potent cancer killer in 20 independent laboratory tests, yet no double-blind clinical trials–the typical benchmark mainstream doctors and journals use to judge a treatment’s value–were ever initiated…

A study published in the Journal of Natural Products, following a recent study conducted at Catholic University of South Korea stated that one chemical in Graviola was found to selectively kill colon cancer cells at “10,000 times the potency of (the commonly used chemotherapy drug) Adriamycin…”

The most significant part of the Catholic University of South Korea report is that Graviola was shown to selectively target the cancer cells, leaving healthy cells untouched. Unlike chemotherapy, which indiscriminately targets all actively reproducing cells (such as stomach and hair cells), causing the often devastating side effects of nausea and hair loss in cancer patients.

A study at Purdue University recently found that leaves from the Graviola tree killed cancer cells among six human cell lines and were especially effective against prostate, pancreatic and lung cancers. Seven years of silence broken–it’s finally here!

A limited supply of Graviola extract, grown and harvested by indigenous people in Brazil, is finally available in America.

The full Graviola Story–including where you can get it and how to use it–is included in Beyond Chemotherapy: New Cancer Killers, Safe as Mother’s Milk, a Health Sciences Institute FREE special bonus report on natural substances that will effectively revolutionize the fight against cancer. This crucial report (along with five more FREE reports) is yours ABSOLUTELY FREE with a new membership to the Health Sciences Institute. It’s just one example of how absolutely vital each report from the Institute can be to your life and those of your loved ones.

From breakthrough cancer and heart research and revolutionary Amazon Rainforest herbology to world-leading anti-aging research and nutritional medicine, every monthly Health Sciences Institute Member’s Alert puts in your hands today cures the rest of America –including your own doctor (!)–is likely to find out only ten years from now.

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